FIG 3.

Uptake of [¹⁴C]Ile. Cells were grown in TSS glucose-ammonium minimal medium, and uptake of [¹⁴C]Ile was measured as described in Materials and Methods at 26°C (A, B, C, and E) or 37°C (D). (A) SMY (wild-type), BB3050 (braB brnQ), BB2913 (bcaP brnQ), BB3055 (bcaP braB), BB3051 (bcaP braB brnQ), and BB3475 (azlB azlCD braB bcaP); (B and C) BB3050 (braB brnQ); (D) BB2913 (bcaP brnQ); (E) BB3475 (azlB azlCD braB bcaP). A 100-fold excess (1 mM) of competing amino acids was added, as indicated, in panels B to E.