TABLE 1.
B. subtilis strains used in this study
| Strain | Genotype or description | Source and/or referencea |
|---|---|---|
| SMY | Prototroph | 28 |
| CU4138 | leuB84::Tn917 trpC2 | 37* |
| CU4139 | liv-1-82::Tn917 trpC2 | 37* |
| QB943 KIT-4 | ilvA1 pyrD1 thyA1 thyB1 trpC2 | 53* |
| PS251 | codY::(erm::spc) trpC2 | P. Serror |
| BB274 | ΔazlB2 (in frame) | 31 |
| BB284 | Δ(azlCD brnQ)::neo | 31 |
| BB2505 | ΔamyE::[erm Φ(bcaP283-lacZ)] lacA::tet | 22 |
| BB2511 | ΔamyE::spc lacA::tet | 27 |
| BB2676 | ΔamyE::[erm Φ(dppA-lacZ)] lacA::tet | 27 |
| BB2726 | ΔbcaP::spc | SMY × pBB1488 |
| BB2770 | ΔamyE::[erm Φ(ybgE292-lacZ)] lacA::tet | 23 |
| BB3046 | ΔbraB::cat | SMY × pBB1586 |
| BB3088 | ΔazlCD | SMY × pBB1595 |
| BB3125 | ΔamyE::[erm Φ(ptb-lacZ)] lacA::tet | BB2511 × pBB1598 |
| BB3165 | bcaPp14 | SMY × pBB1605 |
| BB3198 | bcaPp10/14 | BB3165 × pBB1605 |
a
*, strains obtained from the Bacillus Genetic Stock Center. × indicates transformation by plasmid DNA.