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Oligonucleotide primers used in this study
| Primer | Sequence (5′–3′)a | Specificity |
|---|---|---|
| Forward | ||
| oBB244 | GACAGGGATCCATTCATGTGAATGG | bcaP |
| oBB330 | AAAAAGAATTCACTAGTAACTGCAGTGCATCCAAAGCAC | bcaP |
| oBB396 | CCATCTCTAGAACAGGAACTGTTTC | braB |
| oBB426 | TACAATCTAGATAAATATGGCCTTG | ptb |
| Reverse | ||
| oBB245 | TCATTAAGCTTCAACTCCCGATC | bcaP |
| oBB397 | ATAACGGTACCGTTCTGTGACATTAAC | braB |
| oBB296 | CTGTAAAGCTTCTCTCTTTATCAAAAGG | bcaP |
| oBB427 | GTTACAAGCTTTCTTGTTTCGACTC | ptb |
Restriction sites are underlined.
