TABLE 4.
Role of ILV uptake in the activation of the ptb-lacZ fusiona
| Strain | Relevant genotype | Addition(s) to the medium | β-Galactosidase activity (Miller units) |
|---|---|---|---|
| BB3125 | Wild type | None | 0.22 |
| Ile | 56.4 | ||
| Val | 40.5 | ||
| Leu | 2.44 | ||
| Ile + 13 aa | 12.9 | ||
| BB3143 | bcaP braB brnQ | None | 0.22 |
| Ile | 10.2 | ||
| Val | 3.32 | ||
| Leu | 1.07 | ||
| Ile + 13 aa | 0.80 | ||
| BB3246 | bcaP brnQ | Ile | 35.8 |
| Val | 12.5 | ||
| BB3250 | braB brnQ | Ile | 44.7 |
| Val | 16.4 | ||
| BB3290 | bcaP braB | Ile | 25.9 |
| Val | 8.78 | ||
| BB3291 | azlB2 bcaP braB | Ile | 108.4 |
| Val | 46.9 | ||
| Ile + 13 aa | 36.5 | ||
| BB3285 | bcaPp10/14 | Ile | 114.0 |
| Val | 68.8 | ||
| Ile + 13 aa | 21.3 |
a
Cells were grown in TSS glucose-ammonium medium with or without Ile, Leu, or Val (200 μg/ml) and a mixture of 13 amino acids. The β-galactosidase specific activity was assayed and expressed in Miller units. All values are averages from at least two experiments, and the mean errors did not exceed 30%.