FIG 3.

Mutations in the chromosome upstream of STM4195 restored growth to a panE ilvC strain when grown in the presence of CAA. (A) The open reading frame organization in the relevant genomic region is depicted. (B) The sequence upstream of the STM4195 coding region has been expanded. The predicted −10/−35 promoter regions are underlined. Lowercase letters depict the regulatory binding site for the small RNA repressor, GcvB (41). Allele STM4195p1 contains an IS10-R insertion, indicated by the inverted triangle, inserted 24 bp upstream of the STM4195 start codon (#). The coding sequence of the transposase gene encoded by IS10-R is indicated, as is the promoter reading out of the IS10-R coding region (pOUT). The double-underlined region indicates the 9-bp insertion site recognition sequence that is found duplicated at both ends of the IS10-R insertion (31). The position of allele STM4195p3 (C to T) is denoted with an asterisk and is in the predicted −10 region, 76 bp upstream of the STM4195 start codon.