TABLE 3.
PipA interaction targets determined by immunoprecipitation and mass spectrometry analysis
| Target | Fold enrichment (LSB450 vs. 168)a | Function(s) | Transcriptional regulation |
|---|---|---|---|
| KatE | 7.75 | Catalase 2, detoxification of hydrogen peroxide | σB |
| RbsT | 6.67 | PP2C activator, protein serine kinase, control of σB activity | |
| YdaP | 4 | Putative pyruvate oxidase | σB |
| McpA | 3.25 | Methyl-accepting chemotaxis protein, control of chemotaxis | σD |
| YtpP | 3 | Unknown, similar to thioredoxin H1 | Spx |
| RbfA | 2.47 | Ribosome-binding factor A | Stringent response |
| YorD | 2.33 | Unknown, SPβ-derived stress response protein | AbrB |
| LytB | 2.31 | Modifier protein of major autolysin LytC | σD, YvrGHb, SlrR |
| McpB | 2.27 | Methyl-accepting chemotaxis protein, control of chemotaxis | σD |
| YolA | 2.2 | Unknown, SPβ-derived protein | AbrB |
| RsbV | 2.1 | Anti-anti-σB: control of σB activity | |
| RplL | 1.98 | Ribosomal protein L12 (BL9) | Stringent response |
| YabO | 1.93 | Unknown, similar to heat shock protein Hsp15 | |
| YhdF | 1.9 | Unknown, similar to glucose 1-dehydrogenase | σB |
| HemAT | 1.89 | Soluble chemotaxis receptor, heme-containing O2 sensor protein | σD |
| Spo0F | 1.89 | Phosphotransferase of the sporulation initiation phosphorelay | Spo0A, σH |
| FliD | 1.88 | Filament cap, serves as an extracytoplasmic chaperone required for polymerization of flagellin into the helical filament | σD |
| YabJ | 1.78 | 2-Iminobutanoate/2-iminopropanoate deaminase, required for regulation of purine biosynthesis | PurR |
a
Values are the averages from two experiments. Protein functions and transcriptional regulation are those ascribed in the Subtiwiki (http://www.subtiwiki.uni-goettingen.de) database.