| Reagents | Volume | Final concentration |
| 0.5 M EDTA, pH 8.0 | 0.1 ml | 1 mM |
| 1 M NaOAc, pH6.1 | 25 ml | 0.5 M |
| 1 M Tris-HCl, pH 8.5 | 0.5 ml | 10 mM |
| H2O | 24.4 ml | |
| Total | 50 ml |
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