| 95°C | 1 min 15″ |
| 55°C | 10″ |
| 68°C | 2 min |
| 95°C | 15″ |
| 65°C | 10″ |
| 68°C | 2 min |
| Repeat the last 3 steps another 14 times (16 cycles total) | |
| 68°C | 5 min |
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