| Invitrogen 1st strand buffer | 7.5μl |
| dNTPs mix | 1.9μl |
| Sorbitol/Trehalose mix | 7.5μl |
| DTT (100mM) | 1.9μl |
| Betaine (5M) | 5.6μl |
| SuperScript III RT (200U/μl) | 4μl |
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