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. Author manuscript; available in PMC: 2015 Sep 24.
Published in final edited form as: Nat Commun. 2015 Mar 24;6:6448. doi: 10.1038/ncomms7448

Figure 2. Darifenacin attenuates odor-induced Ca2+ signals of dissociated OSNs in WT but not in M3-R KO mice.

Figure 2

(A) A single cell from an M3-R+/+ mouse was stimulated by a mixture of 10 odorants each at a concentration of 10 μM with or without 0.1 μM darifenacin. The Ca2+ signals were monitored as fluorometric ratios (Fluo-4/Fura-red). KCl serves as a positive control. (B) darifenacin significantly reduced odor-induced Ca2+ signals (two-tailed paired t-test; n = 36 pairs). (C) A single cell from an M3-R-/- mouse was stimulated by a mixture of 10 odorants each at 10 μM with or without 0.1 μM darifenacin. (D) darifenacin failed to attenuate odor-mediated responses of OSNs from M3-R-/- mice (two-tailed paired t-test, n = 62 pairs).