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. Author manuscript; available in PMC: 2015 Sep 24.
Published in final edited form as: Nat Commun. 2015 Mar 24;6:6448. doi: 10.1038/ncomms7448

Figure 5. Possible mechanisms for M3-R-mediated regulation of OR activation.

Figure 5

(A) M3-R could regulate OR activation through (1) the interaction of the two receptors promotes odor-induced OR activation, or (2) the potentiation is the result of downstream crosstalk between OR-mediated cAMP signaling and M3-R-mediated PLC-IP3 signaling, independent of physical interactions. (B) cAMP responses of HEK293T cells expressing OR-S6, M3-R and RTP1S challenged by nonanedioic acid (OR-S6 ligand) in the presence of 5μM PLC inhibitor U73122 or vehicle DMSO. U73122 showed no effect on OR-S6 response with M3-R coexpression (Two-way ANOVA, p=0.20). (C) 5μM PLC inhibitor U73122 abolishes the ER Ca2+ response, demonstrating effective PLC inhibition.