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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Mass Spectrom. 2015 Feb;50(2):418–426. doi: 10.1002/jms.3548

Figure 4.

Figure 4

A demonstration of the process of covalently binding a radical site to a peptide through ion/ion reactions. (a) Ion/ion reaction of the doubly protonated peptide RARARAA (denoted as M) and [SNVazo68-H]−, (b) CID of the resulting electrostatic complex, (c) CID of the remaining electrostatic complex to drive the sulfo-NHS chemistry, and (d) fragmentation of the covalently modified radical peptide. (e) The fragmentation of SNVazo68 into SNA and B is provided. The lightning bolt image indicates the precursor ion for each CID experiment.