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The process of covalently binding a radical site to peptide. (a) Ion/ion reaction between doubly protonated RARARAA (denoted as M) and IBSN; (b) CID of the reaction complex; (c) UVPD of the modified peptide; (d) fragmentation of the modified radical peptide. (e) The C-I bond cleavage via UVPD of IBSN is provided. The lightning bolt image indicates the precursor ion of CID experiments. The star symbol indicates the precursor ion in UVPD experiments.
