Table 1.
Target genes and details of primers and assays used with RT-qPCR analysis. Ta is annealing temperature; PCR efficiency represents the ‘E’ value.
| Gene | Accession number | Primer direction | Primer sequence (5′–3′) | Ta (°C) | E | Product size (bp) | |
|---|---|---|---|---|---|---|---|
| Peroxisome proliferator-activated receptor α | ppara | FJ849065 | Sense | GGGAAAGAGCAGCACGAG | 62.0 | 2.032 | 105 |
| Antisense | GCGTGCTTTGGCTTTGTT | ||||||
| Acyl coA-oxidase | acox1 | CF660510 | Sense | ACAGCACAGCAAGAGCAATG | 59.0 | 1.971 | 104 |
| Antisense | ACAGAGTGGACAGCCGTATC | ||||||
| Thiolase | acaa1 | Seea | Sense | TTGCCTGTGGTGTGGAG | 59.0 | 2.200 | 90 |
| Antisense | CAACATTCTCTGAGGTTATTCC | ||||||
| Cytochrome P450 4 | cyp4 | GU046698 | Sense | TTGACCTCTGCCACTTG | 57.0 | 2.110 | 138 |
| Antisense | CTGATAACTTCCGCTGTATG | ||||||
| Lipoprotein lipase | lpl | FJ716101 | Sense | TTGGGTTACAGTCTTGGTGCTC | 62.0 | 2.110 | 104 |
| Antisense | AAAGGGCATCATCGGGAGAAAG | ||||||
| Apolipoprotein A1 | apoa1 | AJ308993 | Sense | GCCGAAGAAGGTGAAGC | 57.0 | 2.012 | 82 |
| Antisense | GGTGGCAAGGAAGAAAGG | ||||||
| Sterol 27α hydroxylase | cyp27a | CF660988 | Sense | GAGCCACGAAAGTTCAAACC | 56.0 | 2.012 | 88 |
| Antisense | CATCTCCAGTTCAGCAATGC | ||||||
| Cu,Zn superoxide dismutase | sod1 | CA964628 | Sense | GGAATACTCGGTCATTGG | 54.0 | 2.036 | 100 |
| Antisense | ACTGAGTGATGCCTATAAC | ||||||
| Cytochrome P450 2 K | cyp2k | GU046696 | Sense | GCTCTTCCTGTTCTTC | 60.0 | 2.070 | 103 |
| Antisense | TGTGACTTCTACTACTC | ||||||
| Cytochrome P450 3 A | cyp3a | GU046697 | Sense | CCAAGGACCACAAGAAGAAG | 60.0 | 1.921 | 159 |
| Antisense | AGCCGCCGAAGATGAAG | ||||||
| Glutathione S-transferase α | gsta | DQ411310 | Sense | TACAATACTTTCACGCTTTCCC | 61.5 | 1.979 | 149 |
| Antisense | GGCTCAACACCTCCTTCAC | ||||||
| Glutathione S-transferase π | gstp | DQ411313 | Sense | GTCCTTTGCTCTGCCTCTCTG | 60.5 | 2.103 | 141 |
| Antisense | TTACTGCTTGCCATTGCCATTG | ||||||
| Multidrug resistance 1 (ATP-binding cassette sub-family B member 1) | mdr1(abcb1) | AY999964 | Sense | TTGCGGCTGTGGGAAGAG | 58.5 | 2.104 | 109 |
| Antisense | GTGGATGTTCAGTTGCTTTGTG | ||||||
| MDR related protein 2 | mrp2(abcb1) | AY679169 | Sense | TTCGGCTCTAATCTGGATG | 58.5 | 2.080 | 149 |
| Antisense | CTCACCCGCTGTTTCTG | ||||||
| Ribosomal protein 8 | rpl8 | See (Bickley et al., 2009) | Sense | CTCCGTCTTCAAAGCCCATGT | 60.0 | 2.140 | N/A |
| Antisense | TCCTTCACGATCCCCTTGATG | ||||||