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. Author manuscript; available in PMC: 2015 Mar 25.
Published in final edited form as: Biol Chem. 2011 May;392(5):483–489. doi: 10.1515/BC.2011.044

Figure 2.

Figure 2

Determination of the secondary rate constant (kass) of staphopains inhibition by GST-SCCA1.

Constant amounts of staphopain A (panel A) and staphopain B (panel B) were added to samples containing increasing concentrations of GST-SCCA-1 and a fixed amount of substrate. The samples were mixed immediately and the time dependent increase in fluorescence due to substrate hydrolysis was recorded (insets). The data were analyzed by the progress curve method and obtained values of kobs were plotted against inhibitor concentration in the sample (panels A and B). The secondary rate constant was determined by linear regression to the data points and corrected for substrate competition and the stoichiometry factor. The data represent results from triplicate experiments±SD.