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Detection of the SDS-stable GST-SCCA1-SspB inhibitory complex.
To identify the SDS-stable complex of GST-SCCA1-SspB, increasing concentrations of SspB were incubated together with a constant amount of GST-SCCA1 resulting in molar ratios of the inhibitor to the enzyme in the range of 1:0.025–1:2.5. (A) Coomasie G-250-stained gels. (B) Western blot analysis using the mouse monoclonal anti-SspB antibody.
