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. Author manuscript; available in PMC: 2015 Mar 25.
Published in final edited form as: Nat Protoc. 2014 Aug 14;9(9):2135–2146. doi: 10.1038/nprot.2014.143

Figure 3. Typical results seen at step 12 following the sedimentation assay of PFFs.

Figure 3

PFFs were prepared as described in steps 1–12 and the supernatant and pellet resolved by SDS-PAGE and stained with Coomassie Blue. This batch of PFFs had approximately equivalent amounts of α-syn in the supernatant and pellet was efficient at seeding aggregates from endogenous α-syn in primary neurons.