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. Author manuscript; available in PMC: 2015 Mar 25.
Published in final edited form as: Nat Protoc. 2014 Aug 14;9(9):2135–2146. doi: 10.1038/nprot.2014.143

Figure 7. Typical immunoblot results seen following sequential extraction of neurons in 1% Tx-100 followed by SDS.

Figure 7

A. Immunoblotting was performed using Syn1, an antibody that recognizes total α-syn. In control neurons, α-syn was completely extractable in Tx-100. In PFF treated neurons, there was a decrease in Tx-100 soluble α-syn, and increase in SDS-soluble α-syn. B. Immunoblots of SDS extracts (Tx-100 insoluble) using mAB81a that recognizes p-α-syn. There was no p-α-syn in control neurons but a substantial amount of p-α-syn in PFF treated neurons.