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. 2015 Mar 17;16(1):199. doi: 10.1186/s12864-015-1421-8

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© Ho et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of thiourea improved the typeability of Dnd ⁺ strains by PFGE. a: PFGE gel obtained by performing PFGE run without the addition of thiourea. b: PFGE gel obtained by performing PFGE run with the addition of thiourea to the agarose gel and running buffer. Lane M: XbaI digested H9812 DNA marker; lanes S1-S7, VTEC isolates from pigs; lanes A1-A3, clinical isolates obtained from medical center A; lanes B1-B2, clinical isolates obtained from medical center B; C, control E. coli isolate which is typeable with and without the addition of thiourea (a and b). Gel images were captured using GelDoc (BioRad, Hercules, CA) digital gel documentation system.