Figure 4.

Slit1 triggers Netrin-1 attraction for hippocampal neurons on PLL-laminin-coated substrates. (A) Last brighfield images of typical growing axons on PLL-laminin-coated microwells. The growing neurite (yellow line), the initial orientation (dark dotted line), and the angle turned (rotating arrow) are shown. Bar, 20 μm. (B) Scatter plot of the angle turned versus the initial position (x). (C) The mean velocity (±SEM). *P < 0.05, **P < 0.01, Mann–Whitney test in which each condition is compared to the control. (D) The mean angle turned (β) (±SEM) for axons in the different conditions, for initial positions between 300 and 700 μm. Statistical differences are indicated *P < 0.05, **P < 0.01, Kruskal Wallis test with Dunn’s correction.