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. 2015 Mar 3;15:57. doi: 10.1186/s12866-015-0389-5

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© Mao et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Screening and analyses of the positive transformants. A: Antimicrobial activity screening of the positive transformants of Pichia pastoris GAPMP1102. 1–30: the fermentation supernatants of the different transformants of Pichia pastoris GAPMP1102; 31: the fermentation supernatant of Pichia pastoris X-33; 32: 0.5 μg ampicillin. B: Tricine-SDS-PAGE analyses of the different transformants with positive antimicrobial activities. M: A total of 5 μl of protein molecular weight marker (from top to bottom: 40, 25, 15, 10, 4.6 and 1.7 kDa); Lanes 1–7: Totals of 10 μl fermentation supernatants of different transformants of Pichia pastoris GAPMP1102. The arrow indicates rMP1102.