Table 1.
Productivity related parameters of Pichia pastoris GAPMP1102 for fed-batch fermentation under different pH conditions
| pH 5.0 | pH 5.5 | pH 6.0 | pH 6.5 | pH 7.0 | |
|---|---|---|---|---|---|
| Total protein (mg/l) | 807.42 ± 26.35 | 913.28 ± 22.94 | 1076.99 ± 35.86 | 1213.64 ± 42.68 | 1127.38 ± 37.62 |
| rMP1102 (mg/l) | 367.59 ± 13.51 | 423.62 ± 18.27 | 486.35 ± 17.96 | 538.17 ± 21.32 | 503.92 ± 19.85 |
| Activity (AU/ml × 10000) | 3.84 ± 0.31 | 5.12 ± 0.28 | 10.24 ± 0.36 | 15.36 ± 0.43 | 10.24 ± 0.37 |
| Max cell wet weight (g/l) | 341.2 ± 19.41 | 378.5 ± 17.13 | 410.7 ± 16.29 | 424.9 ± 25.81 | 402.6 ± 21.74 |
| Specific cell growth rate (μ) | 0.188 | 0.189 | 0.208 | 0.224 | 0.198 |
| Productivity (AU/mg rMP1102) | 104464 | 120863 | 210548 | 285412 | 203207 |