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. Author manuscript; available in PMC: 2015 Mar 25.
Published in final edited form as: J Immunol. 2014 Oct 22;193(11):5434–5443. doi: 10.4049/jimmunol.1401462

Figure 6. TLR2 agonists directly induce dsDNA-specific IgA production by IgA+ B cells from C4-/- mice.

Figure 6

IgA+ and IgA- splenocytes from wild type and C4-/- mice (n=4 mice/genotype) were cultured for 6 days in complete media alone or in the presence of LPS (20 μg/ml) or Pam3Csk4 (0.5 μg/ml). Supernatants were harvested, diluted 3-fold in PBS, and dsDNA-specific IgA levels were assessed by ELISA. Mean dsDNA-specific IgA values are shown. Asterisks (*) indicate increases in anti-dsDNA IgA levels were significantly greater than than that observed for wild type mice and pound signs (#) indicate significant differences (p<0.05) between treatment conditions.