Table 1.

DNA quantity used for serial dilution library constructions

	Donor #1			Donor #2
Sample Size (10 x bacteria)	Purified dsDNA (ng/ml) 1	DNA for ligation (μg) 2	PCR cycles	Purified dsDNA (ng/ml) 1	DNA for ligation (μg) 2	PCR cycles
10	34.9	1.00	6	30.7	1.00	6
9	4.67	0.41	7	7.17	0.35	7
8	2.68	0.07	8	2.63	0.06	8
7	0.358	<0,04	9	0.356	<0,04	9
6	0.296	<0,03	10	0.228	<0,02	10

¹Genomic dsDNA extracted from indicated number of bacteria.

²Amount of sheared and size purified genomic DNA utilized for ligation with P1 and P2 adaptor oligonucleotides.