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. 2015 Mar 21;16:65. doi: 10.1186/s12891-015-0511-8

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© Ding and Huang; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Histochemical staining of differentiated cells and semi-quantification of the extent of cell differentiation. Both BMSCs and MMSCs were able to differentiate into adipocytes (A, B), osteocytes (C, D), and chondrocytes (E, F), as shown by the accumulation of lipid droplets, proteoglycans and calcium deposits on cell surfaces. However, higher extent of osteogenic differentiation in BMSCs was evidenced by the most positive staining areas through Alizarin Red S assay. Conversely, much higher potential of chondrogenic differentiation in MMSCs was verified by Safranin O staining. Note that each experiment was repeated three times using five different donors. (P < 0.05) (Magnification of microscopy: 20×) (Bar: 50 μm).