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. Author manuscript; available in PMC: 2015 Nov 20.
Published in final edited form as: Nat Commun. 2014 Nov 20;5:5534. doi: 10.1038/ncomms6534

Figure 5. Self-cleavage of the env22 twister ribozyme mutants U5A, U5G, A6G, and G48A.

Figure 5

Cleavage analyzed at 40 μM RNA each strand; 2 mM MgCl2, 100 mM KCl, 30 mM HEPES, pH 7.5, 23 C. R 37-nt ribozyme, S 19-nt substrate; C1 and C2, 14-nt and 5-nt cleavage products. HPLC conditions: Dionex DNAPac colum (4 × 250 mm), 80 C, 1 mL min−1, 0–60% buffer B in 45 min. Buffer A: Tris–HCl (25 mM), urea (6 M), pH 8.0. Buffer B: Tris–HCl (25 mM), urea (6 M), NaClO4 (0.5 M), pH 8.0.