Table 1.
Crystallographic statistics for env22 twister ribozyme
| Crystal | Native | [Ir(NH3)6]3+ soak | Mn2+ soak |
|---|---|---|---|
| Data collection | 24-ID-C | 24-ID-C | 24-ID-C |
| Space group | P6122 | P6122 | P6122 |
| Cell dimensions | |||
| a, b, c (Å) | 64.1, 64.1, 578.8 | 64.0, 64.0, 579.8 | 64.3, 64.3, 585.0 |
| α, β, γ (°) | 90, 90, 120 | 90, 90, 120 | 90, 90, 120 |
| Peak | |||
| Wavelength (Å) | 0.9792 | 1.1052 | 1.7712 |
| Resolution (Å) | 64.3-2.89 (3.04-2.89)* | 50.0-3.70 (3.76-3.70) | 50-3.2 (3.31-3.2) |
| Rpim | 0.041(0.748) | 0.045(0.26) | 0.055 (0.436) |
| I/σI | 19.6 (1.2) | 32 (3.0) | 15.8 (0.7) |
| Completeness (%) | 99.9 (99.6) | 99.8 (99.9) | 95.8 (71.3) |
| Redundancy | 11.9 (12.7) | 14.3 (12.9) | 13.2 (7.4) |
| Refinement | |||
| Resolution (Å) | 55.5-2.89 | 48.7-3.2 | |
| No. reflections | 17191 | 21084 | |
| Rwork/Rfree | 0.18/0.22 | 0.23/0.28 | |
| No. atoms | |||
| RNA | 3541 | 3541 | |
| Cations | 27 | 26 | |
| Water | 83 | 4 | |
| B-factors | |||
| RNA | 105.8 | 118.9 | |
| Cations | 93.2 | 113.3 | |
| Water | 99.5 | 108.4 | |
| R.m.s deviations | |||
| Bond lengths (Å) | 0.006 | 22 | 0.008 |
| Bond angles (°) | 1.335 | 1.136 | |
*
Values for the highest-resolution shell are in parentheses.