Figure 2.

Age-related changes in expression of dynactinP-50 (DynP50) and β-amyloid precursor protein (βAPP). Dynactin-P50 and βAPP were detected by immunofluorescence in tissue sections from hippocampus at the level of the lateral geniculate nucleus from 6 neurologically normal individuals across an age span of 16–80 years. (A) Representative immunofluorescent images from the six individuals; blue represents DAPI staining of cellular DNA, red represents dynactinP-50, green represents βAPP, and yellow represents colocalization of dynactinP-50 and βAPP. Images were digitized at 20× magnification. Scale bar = 20 μm. Quantitation of dynactinP-50 (B) and βAPP (C) immunofluorescence intensity was obtained by thresholding gray-scale images and integrating pixels as described in Materials and Methods section. Values reflect the mean of 6 images per specimen.