Fig. 4.

¹H–¹³C HSQC spectrum (expansion δ_H/δ_C 5.54–4.84/100.0–90.2 ppm, which covers all anomeric cross-peaks) of octasaccharide Lv I obtained from the SEC fractionation displayed at Figure 2. The Fucp units with different sulfation patterns are labeled as the following: A for 2-sulfation, B for 2,4-disulfation, C for 2-sulfation, D for 4-sulfation, A′ for 2-desulfated (non-sulfation) and D′ for the non-reducing end 4-sulfated unit which misses the linked adjacent A unit. The spectrum was recorded at a Varian 600 MHz instrument at 298 K.