Table IV.

Longitudinal spin relaxation (T₁) (in s) of the anomeric ¹H–¹³C cross-peaks of ¹H–¹³C HSQC spectra of Lv I, Lv II and Lv III (Structure 1); and all ¹H–¹³C cross-peaks from ¹H–¹³C HSQC spectra of the oligosaccharide mixture from Structure 2, measured at 298 K

NMR resonance	1H–13C T1 (s)a
Anomeric unit (sulfation type)	Lv I (octasaccharide, Structure 1)
A H1 (2-sulfated)	0.69
B H1 (2,4-disulfated)	0.83
C H1 (2-sulfated)	0.25
D H1 (4-sulfated)	1.07
D′ H1(4-sulfated, non-reducing end)	1.08
A′H1 (unsulfated)	1.13
	Lv II (dodecasaccharide, Structure 1)
A H1 (2-sulfated)	0.51
B H1 (2,4-disulfated)	0.51
C H1 (2-sulfated)	n.d.b
D H1 (4-sulfated)	0.22
D′ H1(4-sulfated, non-reducing end)	0.64
A′ H1(unsulfated)	0.71
	Lv III (hexadecasaccharide, Structure 1)
A H1 (2-sulfated)	0.26
B H1 (2,4-disulfated)	0.41
C H1 (2-sulfated)	0.14
D H1 (4-sulfated)	0.22
D′ H1(4-sulfated, non-reducing end)	0.49
A′ H1(unsulfated)	0.34
Cross-peak within the ring chain	Oligosaccharide mixture (Structure 2)
1H1–13C1	0.35
1H2–13C2	0.38
1H3–13C3	0.44
1H4–13C4	0.43
1H5–13C5	0.35
1H6–13C6	0.66

^aThe T₁ values were measured by 2D NMR ¹H–¹³C inversion-recovery filtered HSQC experiment using a Varian 600 MHz.

^bNot determined due to resonance overlap.