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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Dev Biol. 2014 Aug 4;394(1):110–121. doi: 10.1016/j.ydbio.2014.07.019

Figure 1. RAL-1, exocyst and PAR protein expression in polarized cells.

Figure 1

(A) Schematic of the excretory canal cell (green). The canal cell body and lateral branch are positioned adjacent the posterior pharynx (shaded dark gray). A representative region of posterior canal, depicted at higher magnification in B–D, is indicated by dashed rectangle. (B–D) Lateral view of excretory canal segment in L4 larvae expressing the indicated fusion proteins; arrowheads point towards canal lumen. (E) Schematic of a polarized 1-cell embryo displaying distinct anterior and posterior membrane domains. (F and F′) 1-cell embryo co-expressing SEC-8-mCherry (F) and PAR-6-GFP (F′), which are enriched at the anterior membrane (arrowheads). (G) 1-cell embryo expressing YFP-RAL-1, which localizes uniformly to both anterior (arrowhead) and posterior (arrow) membranes. (H) Schematic of a polarized 8-cell embryo with distinct contacted and contact-free cell surfaces; the germline precursor cell (asterisk) is unpolarized. (I and I′) 8-cell embryo co-expressing SEC-8-mCh (I) and PAR-6-GFP (I′), which are enriched at contact-free surfaces (arrowheads). (J) 8-cell embryo expressing YFP-RAL-1, which localizes uniformly to contact-free (arrowhead) and contacted (arrow) surfaces. In this figure and all subsequent figures, embryos and larvae are oriented anterior to the left, and whole embryos are ~50 μm in length. Scale bars in B–D are 10 μm.