Fig 5.

Serum levels of lipids, inflammation and disease markers of AL- and RF-fed mice. Blood was collected and serum separated for analysis every 3 hrs around the circadian cycle (triglycerides, cholesterol, IL-6, ALT and AST) or at mid-day and mid-night (CRP, PAI-1) from mice fed either AL (solid black line and columns) or RF (dashed grey line and grey columns). For triglycerides, food availability during RF is marked by the crosshatched box. Protein and lipid levels were determined by ELISA. For total daily levels, all time-points were averaged. The grey and black bars designate the subjective day and night, respectively. Values are means ± S.E., n = 3 for each time-point in each group. Asterisk denotes significant difference (P < 0.05).