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Mouse foetal NSCs were seeded and cultured on day 0 ( × 200; A), forming a few neurospheres on day 3 ( × 100; B) and a large amount of the neurospheres on days ( × 100; C). Some became matured neurosphere as seen at high magnification ( × 200 and 400; D and E, respectively). Most of the neurospheres showed nestin-staining to be positive ( × 400; F).
