Fig 3.

Nucleoside transporters and cADPR transportation. (A) RT-PCR detected significant gene expression for CNT1, CNT3, ENT1, ENT2, BST-1 and Cx43. No significant CD38 gene expression was detected in human MSCs. CNT: concentrative nucleoside transporter; ENT: equilibrative nucleoside transporter; BST-1: bone marrow stroma antigen-1; Cx43: connexin 43. (B) Spontaneous Ca²⁺_i oscillations in a representative cell. (C) cADPR had no significant effect on Ca²⁺_i oscillations in the cell pre-treated with 10 nM NBMPR (nitrobenzylthioinosine). Similar results were obtained in a total of 10 cells. (D) The nucleoside transporter inhibitor dypyridamole (100 nM) prevented cADPR effect. (E) Mean values for the effects of 50 μM cADPR (n = 36), 10 nM NBMPR plus 50 μM cADPR (n = 10) and 100 nM dipyridamole plus 50 μM cADPR (n = 11) on the frequencies of Ca²⁺_i oscillations. *P < 0.05 versus before cADPR.