Fig 4.

TRPM2 channel and cADPR in human MSCs. (A) Cyclic ADP ribose increased frequency of Ca²⁺_i oscillations in a representative cell. (B) Cyclic ADP ribose had no significant effect on Ca²⁺_i oscillations in the cell pre-treated with the TRMP2 channel inhibitor clotrimazole. (C) Econazole prevented cADPR effect on Ca²⁺_i oscillations. (D) Mean values for the effects of 50 μM cADPR (n = 36), 10 μM clotrimazole plus 50 μM cADPR (n = 23) and 10 μM econazole plus 50 μM cADPR (n = 26) on frequency of Ca²⁺_i oscillations. *P < 0.05 versus before cADPR. (E) RT-PCR revealed the expression of full length isoform of TRPM2 (TRPM2-L) gene. (F) Western blot analysis showed TRPM2 channel protein in human MSCs from passages 2, 5 and 8.