Fig 4.
Migration capacity of hCMPCs is enhanced by hypoxia. hCMPCs secrete factors under hypoxia that improve the migration. (A) Representative pictures of cell migration after 4 hrs in scratch assay; hCMPCs migrate faster under hypoxia than under normoxia. (B) Quantification of migration in (A). (C) 1% O2-CM induced more migration than 20% O2-CM when the scratch assay was performed in 20% O2. (D) A Boyden chamber assay shows increased migration of hCMPCs towards 1% O2-CM. The number of migrated cells was normalized to unconditioned medium. (E) Gene expression of remodelers of ECM after 1 day of culturing was not significant influenced by hypoxia, except for TSP-2 mRNA expression which was increased under hypoxia. β-actin was measured as a housekeeping gene, and data were normalized to day 0 expression. *Significant difference between normoxia and hypoxia.