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. Author manuscript; available in PMC: 2015 Mar 25.
Published in final edited form as: Hum Mutat. 2014 Dec;35(12):1506–1513. doi: 10.1002/humu.22701

Figure 2.

Figure 2

Immunocytochemical analysis of HeLa cells transiently transfected with wild-type or mutant cochlins. Cochlin localization was detected by immunostaining with an Alexa 555-conjugated anti-HA antibody (red). The ER and Golgi complex were immunostained with Alexa 488 (green) using anti-KDEL and anti-Golgin-97 antibodies, respectively. Nuclei were counterstained with DAPI (cyan). Wild-type, p.G87W, p.P89H, p.V104del, p.I109T, p.A119T, and p.F121S each colocalized with the ER and Golgi complex markers, but both p.C162Y and p.A487P did not colocalize with the Golgi complex markers, indicating lack of transport of these mutant cochlins from ER to the Golgi apparatus.