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. Author manuscript; available in PMC: 2015 Mar 25.
Published in final edited form as: Hum Mutat. 2014 Dec;35(12):1506–1513. doi: 10.1002/humu.22701

Figure 4.

Figure 4

Western blot analysis of cell lysates and culture media from HeLa cells transiently transfected with HA-tagged COCH under non-reducing gel electrophoresis conditions. Additional dimeric cochlin bands were observed for p.V104del, p.I109T, and p.F121S. High-molecular-weight aggregates were detected for p.C162Y and p.A487P mutant cochlins. No aggregates were detected in culture media, and five out of eight mutant cochlins tested were not secreted. The five mutants that failed to be secreted were the same ones that showed dimer and aggregate formation in cell lysates.