Fig 2.
Immature DCs uptake J558-released EXO. (A) Flow cytometric analysis of immature DCs with a panel of antibodies (solid lines) or isotype-matched irrelevant antibodies (dotted lines). (B) Immature DCs (5 × 106 cells) were incubated with J558 tumour cell-released EXO (dotted line) or CFSE-labelled J558 tumour cell-released EXOCFSE (100 mg) (solid line) for 4 hrs and analysed by flow cytometry. (C and D) Immature DCs (5 × 106 cells) were incubated with CFSE-labelled J558 tumour cell-released EXOCFSE (100 mg) (solid line) for different times and analysed by (C) flow cytometry and (D) confocal fluorescence microscopy under differential interference contrast. One representative experiment of two is displayed.