Fig 6.

γ-Secretase complex components were specifically captured by GCB pulldown from human frozen brain material. Solubilized γ-secretase prepared from human brain (frontal cortex) was incubated with 200 nM GCB in the presence (+) or the absence (−) of 10 μM L-685,458 and isolated with SA beads. The captured γ-secretase complex was eluted by 10 mM DTT supplement with 0.01% RapiGest and subjected to Western blotting for the indicated γ-secretase subunit. The density of the bands was calculated as a percentage of a standard (input sample) run on the same gel.