Table 2.
Nanomedicine for Neuro-regeneration 1
| Model | Methods | Mechanism | |
|---|---|---|---|
| Electrospun scaffolds | |||
|
Polyamide w/ Tenascin C peptide103 Ahmed et al |
In vitro - Neural | Neurite outgrowth quantification | Promote outgrowth |
|
PLLA w/laminin100 Koh et al |
In vitro - PC-12 | Neurite outgrowth quantification | Promote attachment |
|
PCL isotropic & anisotropic104 Xie et al |
In vitro -ESCs | Neurite outgrowth quantification, differentiation | Directional cues |
|
PCL isotropic and anisotropic & orthogonal layers105 Xie et al |
In vitro - DRGs | Neurite outgrowth quantification | Directional cues |
|
Polyamide - Tenascin C fabric106 Mieners et al |
In vivo - rat ■ Hemisection |
Neurite outgrowth quantification-3 & 5 weeks | Promote outgrowth |
| Conduits | |||
|
Collagen nanofibrous conduits109 Liu et al |
In vitro - DRGs In vivo - rat ■ Hemisection |
In vitro: Immunostaining In vivo: Immunostaining, neurite outgrowth and axon formation quantification, cell infiltration -10 & 30 days |
Directional cues |
| Self-Assembling Structures | |||
|
RADA16-1 SAPNSs w/SCs or NPCs112 Guo et al |
In vivo - rat ■ Transection |
Staining, axon quantification, lesion volume - 6 weeks | Mimic ECM Neural implantation |
|
RADA16-1 -4G-BMHP1 SAPNSs117 Cigognini et al |
In vivo - rat ■ Contusion |
Gene expression - 3&7 days Lesion volume, staining, histology, BBB scoring - 8 weeks |
Mimic ECM Promote tissue growth |
|
IKVAV PAs111 Tysselin-Mattiace et al |
In vivo - mouse ■ Compression |
Axonal outgrowth quantification - 24h & 9 weeks Modified BBB - 9 weeks |
Mimic ECM Promote outgrowth Suppress astrocytes |
| Combination Self-Assembling and Scaffolding | |||
|
PLGA/PCL microchannel conduits w/RADA16-1-BMHP122 Gelain et al |
In vivo - rat ■ Contusion (chronic) |
Evoked potentials, BBB scoring, Immune response, staining- 24 weeks | Mimic ECM Directional cues Promote tissue growth |
| Conducting Scaffolds | |||
|
PLGA-PPy scaffold Aligned/random130 Lee et al |
In vitro w/electrical stimulation | Neurite outgrowth quantification | Directional cues Electrical cues |
|
Conductive-core sheath PCL-PLA-PPy131 Xie et al |
In vitro w/electrical stimulation | Neurite outgrowth quantification | Directional cues Electrical cues |