Skip to main content
. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Cell Physiol. 2015 Apr;230(4):831–841. doi: 10.1002/jcp.24811

Figure 1. Colocalization of BAG3 with LC3 in autophagosomes.

Figure 1

A. Co-localization of LC3-II and BAG3. T98G cells were transfected with myc-BAG3, and fixed at 48h post-transfection. Cells were co-stained with polyclonal LC3 antibody (green) and monoclonal myc-tag antibody (red). Nuclei were also visualized by DAPI staining (blue). B. T98G cells were transfected with either expression plasmid encoding myc-BAG3 (lane 2) or vector alone (lane1) and subsequently processed for whole-protein extraction at 48h post-transfection for Western blot analysis of autophagy marker, LC3. C. GLI87 primary human glioblastoma cells (grade IV glioblastoma) were either transfected with expression plasmid encoding myc-BAG3 (lane 1) or vector alone (lane1). Cells were harvested at 48h post-transfection and total protein extracts were subjected for western blot analysis of autophagy marker. D. Expression of autophagy-related genes (ATGs) was analyzed by western blot using the same protein extracts that were used in Panel B.