Figure 3.
Blockade of proteasome pathway attenuates anti-apoptotic effect of TSA in NMVM and H9c2 cardiomyoblasts exposed to H/R. Representative images of TUNEL staining of NMVM in A; Blue: DAPI labeled nuclei; Green: FITC-labeled apoptotic nuclei, Red: Cy3 labeled α-sarcomeric actinin, scale bar = 50 µm; B: TUNEL-positive nuclei in NMVM; C: High magnification of stained NMVM, scale bar = 15µm; D: TUNEL-positive nuclei in H9c2 cardiomyoblasts, E: Quantification of TUNEL-positive nuclei *P < 0.0001 vs. N + Vehicle; #P < 0.001 vs. H/R + Vehicle; +P < 0.05 vs. H/R + TSA. Values represent Mean ± SE of five independent experiments; H/R: hypoxia/reoxygenation; TSA: trichostatin A.