Table 1. Size and weight of sar1 grains, florets, and caryopses compared with WT.
Sample | Length (mm) (%) | Width (mm) (%) | Thickness (mm) (%) | Weight (g) (%)d | ||
---|---|---|---|---|---|---|
Mature grainsa | WT | 7.39 ± 0.20 (100) | 3.40 ± 0.13 (100) | 2.33 ± 0.07 (100) | 27.61 ± 0.91 (100) | |
sar1 | 4.91 ± 0.19 (66) | 3.82 ± 0.19 (113) | 2.46 ± 0.08 (106) | 19.26 ± 0.20 (70) | ||
P value | 3.1E-163 | 8.3E-44 | 3.2E-27 | 1.5E-06 | ||
Mature floretsb | WT | 7.69 ± 0.27 (100) | 3.41 ± 0.15 (100) | 1.67 ± 0.13 (100) | - | |
sar1 | 5.55 ± 0.24 (72) | 3.64 ± 0.24 (107) | 1.76 ± 0.14 (105) | - | ||
P value | 7.7E-130 | 1.0E-13 | 1.1E-05 | - | ||
Mature caryopsesa | WT | 5.13 ± 0.12 (100) | 2.96 ± 0.09 (100) | 2.08 ± 0.08 (100) | 22.78 ± 0.90 (100) | |
sar1 | 3.21 ± 0.14 (63) | 3.16 ± 0.15 (107) | 2.22 ± 0.10 (107) | 15.32 ± 0.16 (67) | ||
P value | 2.7E-172 | 4.8E-24 | 1.1E-22 | 3.9E-03 | ||
The glume-cutting experiment | Remaining glumesc | WT | 4.33 ± 0.38 (100) | 3.05 ± 0.18 (100) | 2.21 ± 0.20 (100) | - |
sar1 | 4.33 ± 0.31 (100) | 3.66 ± 0.16 (120) | 2.57 ± 0.21 (117) | - | ||
P value | 9.9E-01 | 1.8E-26 | 1.7E-11 | - | ||
Caryopsesc | WT | 4.90 ± 0.21 (100) | 1.84 ± 0.15 (100) | 1.53 ± 0.15 (100) | - | |
sar1 | 4.98 ± 0.31 (102) | 2.18 ± 0.23 (118) | 1.84 ± 0.23 (120) | - | ||
P value | 1.5E-01 | 1.2E-10 | 5.1E-10 | - |
Means ± standard deviations (SD) are shown. The numbers in parentheses indicate the percentage ratio of sar1 to WT. The symbol ‘-' indicates data were not determined. A Student's t-test was used to generate the P values.
alength, width and thickness were determined from 100-103 completely filled grains or caryopses.
blength, width and thickness were determined from 102-103 mature florets.
cSample number of the glume-cutting experiment is 40.
dThe weight of 100 completely filled grains or caryopses was calculated and converted to 1000-seed weight; data shown in the table represent the mean of three independent measurements.