Table 3. Parameters of microtubule dynamic instability in sar1 root cells compared with WT.
| Sample | Rate (μm/min) (%) | Transition frequencies (events/min) (%) | Percent time in phase (%) (%) | Dynamicity (μm/min) (%) | ||||
|---|---|---|---|---|---|---|---|---|
| Growth | Shrinkage | Rescue | Catastrophe | Growth | Shrinkage | Pause | ||
| WT | 3.76 ± 0.98 (100) | 14.18 ± 4.01 (100) | 3.33 (100) | 0.78 (100) | 66.02 (100) | 15.62 (100) | 18.36 (100) | 5.02 (100) |
| sar1 | 2.82 ± 0.53 (75) | 16.64 ± 5.35 (117) | 3.39 (102) | 0.60 (77) | 66.09 (100) | 13.08 (84) | 20.83 (113) | 4.69 (93) |
| P value | 3.1E-16 | 1.2E-04 | - | - | - | - | - | - |
Means ± SD are shown. The numbers in parentheses indicate the percentage ratio of sar1 to WT. The P values were generated by the Student's t-test. The symbol ‘-' indicates values were not determined. Sample number = 174 microtubules in 55 cells from 4 transgenic WT lines and 187 microtubules in 70 cells from 8 transgenic sar1 lines. Dynamicity is calculated as the sum of the total length grown and shortened divided by the total time of observation for all microtubules.