Table 4. Localization of OsKinesin-13A in cells.
| Anti-OsKinesin-13A Antibody | Preimmune Serum | |
|---|---|---|
| Average Number of Gold Particles | ||
| Particles/cell | 58.17 ± 3.08 | 6.25 ± 0.59 |
| Particles/vesicle | 1.60 ± 0.02 | - |
| Labeling Distribution (in Percent) in Cells | ||
| Vesicles | 52.51 ± 1.65 | 1.08 ± 0.75 |
| Cytoplasm | 36.57 ± 1.74 | 82.84 ± 5.80 |
| Cell membrane | 5.46 ± 0.49 | 0.50 ± 0.50 |
| Golgi stacks | 2.29 ± 0.39 | - |
| Endoplasmic reticulum | 1.74 ± 0.53 | - |
| Other organelles | 1.42 ± 0.42a | 15.58 ± 5.85b |
| Distribution (in Percent) of Labeled Vesicles in Cells | ||
| In the vicinity of cell membranec | 37.96 ± 2.71 | - |
| In the vicinity of Golgi stacksc | 32.04 ± 2.12 | - |
| Other areas in the cytoplasm | 30.00 ± 2.50 | - |
Means ± standard errors of the mean are shown. Sample numbers for immunogold labeling with the anti-OsKinesin-13a antibody and the preimmune serum (negative control) were 41 and 20 root tips cells of WT seedlings, respectively. The symbol ‘-' indicates signals were not detected.
aOrganelles included vacuoles, mitochondria and nuclei.
bOrganelles were nuclei.
cLabeled vesicles located in the area around and near (straight-line distance <1 μm) cell membrane or Golgi stacks.