Table 2. Filtering steps applied to variations called from WES of two families, each with three ASD siblings.

Filtering step	Number of remaining variations
	Family #1	Family #2
Called	185,015	178,706
Covered by 10 or more reads	85,024	82,822
Shared by affected siblings	32,888	33,713
Putatively functional	3,374	3,508
Nonsense	13	12
Frameshift	40	44
Missense	3,321	3,452
GERP > 5	448	417
Novel	4	2

WES, whole-exome sequencing; ASD, autism spectrum disorder; GERP, Genomic Evolutionary Rate Profiling.