Fig. 1.

Urso increases G5G8 and both biliary and FNS in a dose-dependent manner. Male C57BL/6 mice were fed with control, 0.4%TUDCA, or three doses of Urso for 7 days. A: Hepatic levels of ABCG5 and ABCG8 protein expression were determined by immunoblotting. Membrane preparations were blotted for β-actin as controls. B: Hepatobiliary cholesterol secretion rates under basal conditions. Gallbladder was cannulated, and basal bile was collected for 30 min. n = 6 for each group. C: FNS was determined by GC/MS. n = 3 for each group. Data are presented as mean ± SEM. Differences between TUDCA and control were determined by two-tailed t-test. Differences between control and three doses of Urso were determined by one-way ANOVA followed by Dunnett’s tests. * P < 0.05, ** P < 0.01, *** P < 0.001.