Fig. 3.

EZ produced an additive effect for fecal sterol elimination. Male C57BL/6 mice were fed with control, 0.3% Urso, or 0.3% Urso combined with a low or high dose of EZ for 14 days. A: Hepatic levels of ABCG5 and ABCG8 protein expression were determined by immunoblotting. B: Hepatobiliary cholesterol secretion rates under basal conditions. n = 5–7 for each group. C: FNS was determined by GC/MS. n = 7 for each group. Data are presented as mean ± SEM. Differences were determined by one-way ANOVA followed by Bonferroni post hoc tests. * P < 0.05, **** P < 0.0001.