Fig. 5.

EZ reduced intestinal G5G8 and ABCA1. A–D: The mRNA expression for jejunum ABCG5, ABCG8, NPC1L1, and ABCA1 was determined by RT-PCR. n = 6 for each group. E: Immunoblot analysis of jejunum ABCG5, ABCG8, and ABCA1. F: Serum (60 μl) from 4 mice in each group was fractionated by FPLC and analyzed for the distribution of cholesterol among lipoproteins. Horizontal bars indicated elution fractions for lipoproteins. Data are mean ± SEM. Differences were determined by one-way ANOVA followed by Bonferroni post hoc tests. * P < 0.05, ** P < 0.01 *** P < 0.001, **** P < 0.0001.